Incubation:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
Western Blot:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
Control:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
Staining:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
Multiplex Assay:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
Immunofluorescence:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
Luciferase:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
Transfection:Article Title: Identification of A p300-SP1-BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome.
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].Western blotting was conducted on ovarian tissue homogenates and cultured-cell lysates essentially as before [ 10 ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY-P80167, MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY-P80182, MCE), H4K8ac (HY-P80180, MCE), H4K12ac (ab46983, abcam), H4K16ac (HY-P80179, MCE), His- the overlap between gene with increased peaks (ATAC-seq, p < 0.05 ) and gene DHEA-treated mice. (e) Peak plot showing the ATAC-seq peaks (normalized 98147619–98152019) in Ctrl (blue) vs. DHEA-treated (red) mice.. The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict SP1.The peaks exh boxes and red asterisk. (f) A heatmap displays the top six transcription factors with their mRNA expression identified by RNA-seq analysis, and the predict..
Article Title: Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome
Article Snippet: Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].Western blotting was conducted on ovarian tissue homogenates and cultured‐cell lysates essentially as before [ ].. Primary antibodies used included: p300 (ab275378, Abcam), BRD4 (83375S, CST, USA), AR (ab52615, Abcam), Histone3 lysine site 9 acetylated antibody (H3K9ac, ab4441, abcam), H3K14ac (HY‐ P80167 , MCE), H3K18ac (13998T, CST), H3K27ac (8173T, CST), H4K5ac (HY‐ P80182 , MCE), H4K8ac (HY‐ P80180 , MCE), H4K12ac (ab46983, abcam), H4K16ac (HY‐ P80179 , MCE), Histone3 (H3, 17168‐1‐AP, Proteintech), Histone4 (H4, 16047‐1‐AP, Proteintech), α‐SMA (BS70000, Bioworld), CBP (AG1691, Beyotime), Gcn5 ( PA002657 , CUSBIO), Tip60 ( PA006616 , CUSBIO), SRC‐3 (ER65603, HUABO), Col1α (BS1530, Bioworld), SP1 (A19649, ABclonal), and GAPDH (FD0063, FDbio).. Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).Secondary antibodies included goat anti‐rabbit IgG‐HRP (FDR007, FDbio) and goat anti‐mouse IgG‐HRP (FDM007, FDbio).
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